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daily), smooth muscle contractility was restored in the pre-inflammation site and significantly improved in the inflammation site.Ĭonclusion: Colonic smooth muscle contractility is significantly impaired in stenotic Crohn’s-like colitis rats not only in the inflammation site, but in the distended pre-inflammation site. When rats were administered with COX-2 inhibitor NS-398 (5 mg/kg, i. Liquid diet treatment, mimicking exclusive enteral nutrition, completely released mechanical distention, eliminated COX-2 expression and PGE 2 production, and improved smooth muscle contractility especially in the pre-inflammation site. Smooth muscle contractility was suppressed, and expression of cyclo-oxygenase-2 (COX-2) and production of prostaglandin E 2 (PGE 2) were increased not only in the inflammation site but also in the pre-inflammation site. Results: When rats were fed with solid food, TNBS treatment induced localized transmural inflammation with stenosis in the instillation site and marked distention with no inflammation in the pre-inflammation site of the colon. The rats were fed with either regular solid food or exclusively liquid diet. Methods: Crohn’s-like colitis was induced by intracolonic instillation of TNBS (65 mg/kg) in Sprague-Dawley rats. We tested the hypothesis that mechanical stress plays a role in muscle dysfunction in a rat model of Crohn’s-like colitis where inflammatory stenosis leads to mechanical distention in the pre-inflammation site. The mechanisms for muscle dysfunctions are incompletely understood. 4Department of Biochemistry, Cancer Biology, Neuroscience and Pharmacology, Meharry Medical College School of Medicine, Nashville, TN, United Statesīackground and Aims: Gut smooth muscle dysfunctions contribute to symptoms such as abdominal cramping, diarrhea, and constipation in inflammatory bowel disease (IBD).3Department of Pathology, University of Texas Medical Branch, Galveston, TX, United States.

2John Sealy School of Medicine Class of 2025, University of Texas Medical Branch, Galveston, TX, United States.1Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, United States.Johnson 1,2 Ramasatyaveni Geesala 1 Ke Zhang 1 You-Min Lin 1,3 Amosy E.